EFFICACY TESTING OF UNIGROW BIO-FERTILIZER WITH PECHAY BLACK-BEHI VARIETY AS TEST CROP

June 21, 2007

MR. JAIME VISTAR
#1750 Garcia St., New J. P. Rizal
Santiago Vilage, Makati City

Dear Mr. Vistar

This is to submit the results on the efficacy test conducted on Unigrow Bio-fertilizer with Pechay Black-Behi variety as test crop. The test was conducted in Amadeo, Cavite last May to June 2007.

For any additional information you may wish to know concerning the test please do not hesitate to write us.

Very truly yours,

(SGD.)
NORA B. INCIONG, Ph.D.
Researcher
#20 Santan St., Ynchausti Subd.
Malabon, Metro Manila

EFFICACY TESTING OF UNIGROW BIO-FERTILIZER WITH PECHAY BLACK-BEHI VARIETY AS TEST CROP

OBJECTIVES:

1. To determine the effects of Unigrow Bio-Fertilizer on the growth and yield of plants with Pechay Black-Behi variety as the test crop.

EXPERIMENTAL SITE

The testing on the effectiveness of Unigrow Bio-Fertilizer was conducted at Amadeo, Cavite.

DURATION OF THE STUDY:

The test was conducted last May 1 to June 2007.

EXPERIMENTAL DESIGN AND FERTILIZER APPLICATION

A Randomized Complete Block Design (RCBD) was adopted for the test. There were three blocks with each block consisting of six (6) plots. Each plot represented a replicate. Each plot measured 3.0 meter squares. The distance of planting was 25 cm between rows and 25 cm between hills. There were a total of 40 plants per plot. The following treatments were tested.

T1 - Control

T2 - RIF (80-20-20)

Basal application per plot – 42.86 grams 14-14-14
39.0 grams 46-0-0

The chemicals were dissolved in 40 glasses of water and applied at one(1) glass per plant directly to the whole plant and down to the roots at three (3) days after transplanting.

T3 - ½ RIF

Basal application per plot - 21.43 grams 14-14-14
19.5 grams 40-0-0

The chemicals were dissolved in 40 glasses of water and applied at one(1) glass per plant directly to the whole plant and down to the roots at three (3) days after transplanting.

T4 - Unigrow Bio-Fertilizer at one (1) kilogram per hectare.

Six (6) grams of Unigrow Bio-fertilizer was dissolved thoroughly in 6 liters of water. This was used to water more or less evenly T4, T5 and T6 plots.

T5 - RIF + Unigrow Bio-fertilizer at one (1) kilogram per hectare.

The same applications with chemical fertilizers as in T2 and Unigrow Bio-fertilizer as j T4 were followed.

T6 - ½ RIF + Unigrow Bio-fertilizer at onje (1) kilogram per
hectare.

The same applications with chemical fertilizers as in T3 and Unigrow Bio-fertilizer as in T4 were followed.

LAND PREPARATION

An area of approximately 300 square meters including an area for buffer was thoroughly cultivated. Three blocks, each measuring approximately 15m x 3m were laid out. Approximately one-half (1/2) meter was provided between plots to prevent contamination of treatments. Each block was divided into six (6) plots. Each plot measured 3 square meters.

Approximately ½ meters wide of border area surrounding the blocks on four sides was also cultivated to serve as buffer.

SEEDLING PREPARATION AND TRANSPLANTING

Pechay seeds of Black-Behi variety were sown uniformly in a prepared seeded last May 5, 2007. The seedlings were ready for transplanting after 19 days.

One seedling was transplanted per hill in the prepared test plots. Distance of planting was 25 centimeters between rows and 25 cm between hills. A total of 40 hills were present in each plot. Transplanting was done Last May 24, 2007.

CARE AND MANAGEMENT OF THE PLANTS

a. Weed Management

Regular hand weeding and cultivation around the base of pechay plants were done to prevent the growth of weeds.

b. Water Management

Regular watering was done or when necessary to ensure that the soil has enough moisture for the growth of pechay plants.

c. Pesticide Management

The test was conducted inside a fine nylon net sewed like a mosquito net. As a result, there were no severe pest infestation as well as no pesticide or insecticide was sprayed during the conduct of the experiment.

DATA COLLECTION

Twelve (12) pechay plants at the center of each plot were selected and marked as observation plants for height, number and weight of marketable stems with leaves.

STATISTICAL ANALYSIS

All data gathered were tabulated and statistically analyzed following the F-test.

NOTE: INSERT ON NEXT PAGES THE FOLLOWING TABLES RE: LETTER OF JUNE 21, 2007 TO MR. VISTAR

TABLE 1A; TABLE 1B; TABLE 2A; TABLE 2B; TABLE 3A; TABLE 3B; AND TABLE 3C.

DISCUSSION RESULTS

The Unigrow Bio-fertilizer is a pure biological product, very necessary components essential to the growth development of the plants. It promotes regulating functions by cell expansion, extension seed resting and fruit maturity.

The influence of Unigrow Bio-fertilizer on the height of pechay plants (Table 1A, 1B)

Table 1A shows the average height of pechay plants as influenced by the application of the Unigrow Bio-fertilizer. The control plants were the shortest (T1) with an average height of 19.27 centimeters. The application of the Unigrow Bio-fertilizer showed to be effective in improving the growth of pechay plants. The Unigrow Bio-fertilizer at T4 applied after transplanting resulted to significantly taller plants compared with the contol (T4 vs T1). The control had an average of 20.31 cm whereas the T4 reached 21.47 centimeters. The RIF plants reached the average height of 22.42 cm, the ½ RIF reached 21.73 centimeters. The Unigrow Bio-fertilizer plants were statistically similar to ½ RIF plants.

When the Unigrow Bio-fertilizer was applied to plants with the chemical fertilizers as in T5 and T6, the pechay grew taller. The T5 had 26.26 centimeters. The increase due to Unigrow Bio-fertilizer was equivalent to 29.30 percent. In T6, where plants were fertilized with ½ RIF and Unigrow Bio-fertilizer, the increase in plant height due to uUnigrow Bio-fertilizer was 1.42 cm which was approximately 6.53 percent.

The influence of Unigrow Bio-fertilizer on the number of leaves of pechay plants (Table 2A, 2B)

The number of leaves presented in Table 2A are those left after removing the first two (2) undeveloped stalks as well as the rotten stalks due to insect or disease damage. In case of this study, pest or disease damage was avoided since the plants wre harvested at the onset o thrips infestation. The counting included not only the fully developed leaves but also the newly emerging young shoots.

As shown in Table 2A, the pechay plants grown in plots fertilized with the chemical fertilizers as the RIF and ½ RIF, had more leaves than the control plants. The control plants T1 had developed an average of 7.13 leaves per plant. The RIF had 8.10 leaves. The increase in RIF and ½ RIF were equivalent to 35.63% and 13.60% respectively compared with the control plants. These increases were statistically significant. The average number of leaves of the Unigrow Bio-fertilizer applied plants (T4) was 9.33. The increase over the control was equivalent to 30,86% percent.

When the Unigrow Bio-fertilizer was applied to the base of the plants grown in plots fertilized with the RIF and ½ RIF, significant increase in leaf development was observed. Compared to RIF alone (T2), the RIF + Unigrow Bio-fertilizer (T5) had developed an average of 11.80 leaves or stalks. This increase was approximately 22.03%. The ½ RIF + Unigrow Bio-fertilizer (T6) had resulted to an increase of 11.04 leaves which was equivalent to 36.30 percent compared with ½ RIF (T3) only.

The influence of Unigrow B-fertilizer on the weight of pecha plants (Tables 3A, 3B, 3C)

Both the chemical fertilizers and the Unigrow Bio-fertilizer had significantly resulted to bigger plants. Not only did the plants developed more leaves but also weighed even more significantly than the control plants.

In Table 3A, it is shown that the control plants (T1) had an average weight of 52.67 grams per plant. The RIF plant (T2) had 82.67 grams. The increase due to fertilizer application was 30.0 gram or 56.96 percent. The ½ RIF (T3) plants had 71.0 grams compared with the control plant. This was equivalent to 34.80 percent compared with the control.

The Unigrow Bio-fertilizer applied plants (T4) had slightly heavier plants compared with the ½ RIF (T4 vs T3). The Unigrow Bio-fertilizer (T4) fertilized plants were heavier by approximately 15.25 percent compared with ½ RIF plants (T4 vs T3).

When Unigrow Bio-fertilizer was applied as supplement to the chemical fertilizers as in T5 and T6, the plants were more than double in weight compared with the control plants. In T5 vs T1, the increase was 59.61 grams or 113.18 percent.

The effect of Unigrow Bio-fertilizer as supplement to the chemical fertilizers was equivalent to the increase between T5 and T2. In T2, the plants received the chemical fertilizers whereas in T5, the plants received both the chemical and Unigrow Bio-fertilizer. The T2 plants had an average weight of 82.67 grams whereas the T5 plants had 112.28 grams. The increase due to the Unigrow Bio-fertilizer was 29.61 grams or 35.82 percent. In T6 vs T3, the Unigerow Bio-fertilizer had resulted to an increase in weight by 25.23 grams compared with ½ RIF. This increase was 35.82 percent.

The computed yield per hectare (Table 3C) was obtained by multiplying the average weight per plant with an assumption of 102,400 plants per hectare. The number of plants per hectare was based on the assumption that only 80% of the land was utilized for planting while the 20% was occupied for alleys and borders. Distance of planting was 0.25 m between rows and 0.20 cm between plants within the row. Pechay are usually planted in prepared plots of 1 ½ wide and variable lengths depending on farmer’s convenience during watering and cultivation.

SUMMARY AND CONCLUSIONS

1. The pechay plants responded significantly in terms of growth and weight to the application of chemical fertilizers and the Unigrow Bio-fertilizer.

2. The application of chemical fertilizers and Unigrow Bio-fertilizer had improved the growth of the pechay plants by 6.17% in height and 35% in weight.

3. As supplement to the RIF and ½ RIF the Unigrow Bio-fertilizer had resulted to an additional increase in weight by 35.0 percent.

-? 1.5 m ?—

B-1 T4 T1 T5 T6 T7 T3
B-2 T2 T6 T3 T4 T1 T5
B-3 T5 T3 T2 T1 T4 T6
2 m

Field Lay-out for Efficacy Testing of Unigrow Bio-Fertilizer
with Pechay Black-Behi Variety as Test Crop